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KMID : 0545119970070040262
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Journal of Microbiology and Biotechnology
1997 Volume.7 No. 4 p.262 ~ p.266
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Production of 1-Deoxynojirimycin by Streptomyces sp. SID9135
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Paek, Nam Soo
Kang, Dae Jung/Choi, Yong Jin/Lee, Jung Jun/Kim, Tae Han/Kim, Kee Won
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Abstract
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Overproduction of Escherichia coli thiol peroxidase in the periplasmic space was achieved by locating the appropriate gene on a downstream region of the strong T7 promoter. E. coli strain BL21 carrying the recombinant plasmid pSK-TPX was induced by IPTG, lysed, and analyzed by SDS-polyacrylamide gel electrophoresis. A large amount of the overexpressed thiol peroxidase was located in the periplasmic space. A homogeneous thiol peroxidase was obtained from E. coli osmotic shock fluid by simple one-step gel permeation chromatography.
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